Cell Reports Methods
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New articles

Articles below are published ahead of final publication in an issue. Please cite articles in the following format: authors, (year), title, journal, DOI.

September 22, 2022


STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells

  • Albert Blanch-Asensio,
  • Catarina Grandela,
  • Karina O. Brandão,
  • Tessa de Korte,
  • Hailiang Mei,
  • Yavuz Ariyurek,
  • Loukia Yiangou,
  • Mervyn P.H. Mol,
  • Berend J. van Meer,
  • Susan L. Kloet,
  • Christine L. Mummery,
  • Richard P. Davis
First published:September 22, 2022
Blanch-Asensio et al. present a method to integrate large DNA payloads into specific genomic sites of hPSCs. This procedure offers a precise and rapid approach to generate genetically matched panels of hPSC lines with applications in research areas such as disease modeling and synthetic biology.

September 21, 2022


Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation

  • Xiaowen Wang,
  • Christine Delle,
  • Antonis Asiminas,
  • Sonam Akther,
  • Marta Vittani,
  • Peter Brøgger,
  • Peter Kusk,
  • Camilla Trang Vo,
  • Tessa Radovanovic,
  • Ayumu Konno,
  • Hirokazu Hirai,
  • Masahiro Fukuda,
  • Pia Weikop,
  • Steven A. Goldman,
  • Maiken Nedergaard,
  • Hajime Hirase
First published:September 21, 2022
Our ability to study microcirculation longitudinally is restricted by the short in vivo life of injectable fluorescent dyes. Wang et al. describe a method that enables longitudinal visualization of blood plasma in mice after a single injection of AAV viruses, expressing fluorescent secretory albumin-fusion proteins in the liver.

September 20, 2022


Heterologous reporter expression in the planarian Schmidtea mediterranea through somatic mRNA transfection

  • Richard Nelson Hall,
  • Uri Weill,
  • Leonard Drees,
  • Sergio Leal-Ortiz,
  • Hongquan Li,
  • Margarita Khariton,
  • Chew Chai,
  • Yuan Xue,
  • Benyamin Rosental,
  • Stephen R. Quake,
  • Alejandro Sánchez Alvarado,
  • Nicholas A. Melosh,
  • Andrew Z. Fire,
  • Jochen C. Rink,
  • Bo Wang
First published:September 20, 2022
Hall et al. demonstrate luminescence reporter expression from transfected mRNA in the regenerative planarian flatworm Schmidtea mediterranea. This study paves the way for developing additional transgenic techniques in planarians and provides a means to investigate post-transcriptional regulation of gene expression in this previously genetically intractable organism.

Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy

  • Qianli Zhang,
  • Akitoshi Miyamoto,
  • Shin Watanabe,
  • Takao Arimori,
  • Masanori Sakai,
  • Madoka Tomisaki,
  • Tai Kiuchi,
  • Junichi Takagi,
  • Naoki Watanabe
First published:September 20, 2022
Zhang et al. establish a versatile mutagenesis strategy to efficiently increase the dissociation rate of antibodies without compromising binding specificity. Engineered fast-dissociating antibody fragments enable high-density IRIS multiplexed super-resolution imaging, which overcomes the intrinsic sparse labeling problem caused by spatial interference between multiple antibodies in other super-resolution approaches.

SpecSeg is a versatile toolbox that segments neurons and neurites in chronic calcium imaging datasets based on low-frequency cross-spectral power

  • Leander de Kraker,
  • Koen Seignette,
  • Premnath Thamizharasu,
  • Bastijn J.G. van den Boom,
  • Ildefonso Ferreira Pica,
  • Ingo Willuhn,
  • Christiaan N. Levelt,
  • Chris van der Togt
First published:September 20, 2022
de Kraker et al. present SpecSeg, a user-friendly and versatile open-source toolbox for calcium imaging data acquired with miniscopes or multi-photon microscopes. It selects regions of interest (ROIs) around neurons and neurites, performs neuropil correction and signal extraction, and matches ROIs in sequential recordings.