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Cell Stem Cell
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Metastable Pluripotent States in NOD-Mouse-Derived ESCs

      (Cell Stem Cell 16, 566–568; May 7, 2015)
      We became aware from a reader that one of the images included in the Erratum that we published earlier this year closely resembled an image in one of the other figures in the paper. Specifically, the SSEA1 stained colony presented for 129 iPSEpiSC #1K in Figure S10A was the same as the colony presented as SSEA1 staining of NOD-iPSC mES +KP/CH in Figure S1A. Although the two images are not identical, from examination of the relationship between them, we concluded that they do represent the same colony of cells and yet were presented as being distinct.
      We have investigated the basis of this duplication and have determined that it resulted from a mistake in file saving and naming during recording of the data for these experiments. An SSEA1 staining image of a colony of NOD-iPSC mES +KP/CH cells, shown in Figure S1A, was inadvertently saved and mislabeled as an SSEA1 staining of 129 iPSEpiSC #1K. We have examined the original data files related to this overall experiment and have verified based on date/time stamping and laboratory notes that this incorrect file labeling was the basis of the error. We also sent the relevant original data files to the journal editors for verification. Subsequent images saved shortly after the mislabeled one were correctly recorded as 129 iPSEpiSC #1K and we have used a representative image from the correct series to produce a revised version of Figure S10 as shown below. All of the other panels shown in Figure S10 remain the same, and the conclusions of the paper are not affected.
      We are grateful to the reader who brought this unfortunate mistake to our attention and apologize for any confusion caused to our colleagues in the community.

      Linked Article

      • Metastable Pluripotent States in NOD-Mouse-Derived ESCs
          Hanna et al.
        Cell Stem CellMay 7, 2009
        • In Brief
          Embryonic stem cells (ESCs) are isolated from the inner cell mass (ICM) of blastocysts, whereas epiblast stem cells (EpiSCs) are derived from the postimplantation epiblast and display a restricted developmental potential. Here we characterize pluripotent states in the nonobese diabetic (NOD) mouse strain, which prior to this study was considered “nonpermissive” for ESC derivation. We find that NOD stem cells can be stabilized by providing constitutive expression of Klf4 or c-Myc or small molecules that can replace these factors during in vitro reprogramming.
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      • Metastable Pluripotent States in NOD-Mouse-Derived ESCs
          Hanna et al.
        Cell Stem CellMay 07, 2015
        Open Archive