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Cell Stem Cell
This journal offers authors two options (open access or subscription) to publish research

Sep 03, 2015

Volume 17Issue 3p249-372
Open Archive
On the cover: In this issue, Shin et al. (360–372) present an approach for capturing the molecular dynamics of somatic stem cells in vivo using transcriptomic snapshots at single-cell resolution. The cover image is inspired by one of the first motion pictures, “The Horse in Motion,” by Eadweard Muybridge (1830–1904), which resolved a popular debate about whether all four feet of a horse are off the ground at the same time while it's galloping. Muybridge used large glass-plate cameras placed in a line along the edge of a track that were triggered as a horse passed to create a series of images that captured its gait. The cover design was conceived by Jaehoon Shin....
On the cover: In this issue, Shin et al. (360–372) present an approach for capturing the molecular dynamics of somatic stem cells in vivo using transcriptomic snapshots at single-cell resolution. The cover image is inspired by one of the first motion pictures, “The Horse in Motion,” by Eadweard Muybridge (1830–1904), which resolved a popular debate about whether all four feet of a horse are off the ground at the same time while it's galloping. Muybridge used large glass-plate cameras placed in a line along the edge of a track that were triggered as a horse passed to create a series of images that captured its gait. The cover design was conceived by Jaehoon Shin.

In This Issue

Previews

  • Adaption by Rewiring Epigenetic Landscapes

    • Yifei Liu,
    • Andrew Xiao
    Embryonic stem cells (ESCs) generally rely on repressive histone modifications to silence retrotransposons, rather than DNA methylation as in differentiated cells. In this issue of Cell Stem Cell, He et al. (2015) show that Daxx/Atrx repress transposons in ESCs devoid of 5mC, demonstrating dynamic reorganization of epigenetic networks and crosstalk between distinct repressive mechanisms to maintain transposon silencing.
  • Sequencing Single Cells Reveals Sequential Stem Cell States

    • Sten Linnarsson
    Understanding the molecular mechanisms underlying activation of quiescent neural stem cells (NSCs) is complicated by heterogeneity in coexisting NSC pools. Two papers in this issue of Cell Stem Cell (Llorens-Bobadilla et al., 2015; Shin et al., 2015) report sequencing of single NSCs, providing insights into the transition from quiescence to activation and highlighting common themes in NSCs from distinct brain regions.
  • Enlisting hESCs to Interrogate Genetic Variants Associated with Neuropsychiatric Disorders

    • Steven E. Hyman
    Connecting rare genetic variants to neuropsychiatric disease mechanisms remains a significant challenge. In this issue of Cell Stem Cell, Pak et al. (2015) combine gene targeting and stem cell technologies to identify a significant cellular effect of rare penetrant NRXN1 mutations in human neurons, which was found to cause a defect in neurotransmitter release.
  • The Path from Pluripotency to Skeletal Muscle: Developmental Myogenesis Guides the Way

    • Michael Hicks,
    • April Pyle
    Following cues from mouse embryogenesis, Chal et al. (2015) identified key regulators of skeletal myogenesis from mouse and human pluripotent stem cells. Emerging myogenic progenitors were specified to form multinucleated fibers that enabled development of quiescent, satellite cell-like progenitors and a model for Duchenne Muscular Dystrophy.

In Translation

Perspective

Articles

Short Article

  • Humanized Mice Reveal Differential Immunogenicity of Cells Derived from Autologous Induced Pluripotent Stem Cells

    • Tongbiao Zhao,
    • Zhen-ning Zhang,
    • Peter D. Westenskow,
    • Dilyana Todorova,
    • Zheng Hu,
    • Tongxiang Lin,
    • Zhili Rong,
    • Jinchul Kim,
    • Jingjin He,
    • Meiyan Wang,
    • Dennis O. Clegg,
    • Yong-guang Yang,
    • Kun Zhang,
    • Martin Friedlander,
    • Yang Xu
    Patient iPSCs have potential as a renewable source for autologous cell therapy that avoids immune rejection. Using a humanized mouse model with a functional human immune system, Zhao et al. observe differential immune responses to various autologous hiPSC derivatives, including rejection of smooth muscle cells and tolerance to retinal pigmented epithelium.

Resource

  • Single-Cell RNA-Seq with Waterfall Reveals Molecular Cascades underlying Adult Neurogenesis

    • Jaehoon Shin,
    • Daniel A. Berg,
    • Yunhua Zhu,
    • Joseph Y. Shin,
    • Juan Song,
    • Michael A. Bonaguidi,
    • Grigori Enikolopov,
    • David W. Nauen,
    • Kimberly M. Christian,
    • Guo-li Ming,
    • Hongjun Song
    In vivo molecular dynamics of adult stem cells have been elusive. Shin et al. used single-cell RNA-seq and a novel bioinformatic approach named Waterfall to reconstruct somatic stem cell dynamics with unprecedented temporal resolution. The genome-wide molecular transitions they identified suggest commonalities among different somatic stem cell systems.
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