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Developmental Cell
This journal offers authors two options (open access or subscription) to publish research

May 23, 2022

Volume 57Issue 10p1209-1310
On the cover: This image represents three studies in this issue of Developmental Cell that are part of the SpatioTemporal Omics Consortium (STOC): zebrafish embryogenesis spatiotemporal transcriptomic atlas (bottom right), 3D spatiotemporal transcriptomic atlas of developing Drosophila (top right), and cell-type-specific spatial transcriptomic atlas of arabidopsis (left). To learn more about Stereo-seq and its applications, see Wang et al. (pp. 1271–1283), Liu et al. (1284–1298), and Xia et al. (1299–1310). Image credit: Mingjiang Lu, Huifang Xiao....
On the cover: This image represents three studies in this issue of Developmental Cell that are part of the SpatioTemporal Omics Consortium (STOC): zebrafish embryogenesis spatiotemporal transcriptomic atlas (bottom right), 3D spatiotemporal transcriptomic atlas of developing Drosophila (top right), and cell-type-specific spatial transcriptomic atlas of arabidopsis (left). To learn more about Stereo-seq and its applications, see Wang et al. (pp. 1271–1283), Liu et al. (1284–1298), and Xia et al. (1299–1310). Image credit: Mingjiang Lu, Huifang Xiao.

Preview

  • New adventures in spatial transcriptomics

    • Maayan Pour,
    • Itai Yanai
    Complex dynamic processes such as development involve the deployment of gene regulatory pathways that transform the spatial arrangement of cells. Disentangling these genetic programs is at the core of many biological problems. Stereo-seq is a promising spatial transcriptomics method, as demonstrated by three papers in this issue of Developmental Cell, each in a distinct biological context.

Articles

  • Mitochondrial dynamics regulate genome stability via control of caspase-dependent DNA damage

    • Kai Cao,
    • Joel S. Riley,
    • Rosalie Heilig,
    • Alfredo E. Montes-Gómez,
    • Esmee Vringer,
    • Kevin Berthenet,
    • Catherine Cloix,
    • Yassmin Elmasry,
    • David G. Spiller,
    • Gabriel Ichim,
    • Kirsteen J. Campbell,
    • Andrew P. Gilmore,
    • Stephen W.G. Tait
    Open Access
    Sub-lethal apoptotic stress can cause oncogenic DNA damage. Investigating its basis, Cao, Riley, and colleagues report that mitochondrial dysfunction promotes sub-lethal apoptotic stress. Mitochondrial dysfunction causes permeabilization of select mitochondria through mitochondrial fission and pro-apoptotic BAX mitochondrial accumulation. Therefore, mitochondrial function and genome stability are interconnected via sub-lethal apoptotic stress.
  • The myogenesis program drives clonal selection and drug resistance in rhabdomyosarcoma

    • Anand G. Patel,
    • Xiang Chen,
    • Xin Huang,
    • Michael R. Clay,
    • Natalia Komorova,
    • Matthew J. Krasin,
    • Alberto Pappo,
    • Heather Tillman,
    • Brent A. Orr,
    • Justina McEvoy,
    • Brittney Gordon,
    • Kaley Blankenship,
    • Colleen Reilly,
    • Xin Zhou,
    • Jackie L. Norrie,
    • Asa Karlstrom,
    • Jiyang Yu,
    • Dominik Wodarz,
    • Elizabeth Stewart,
    • Michael A. Dyer
    Rhabdomyosarcoma is a pediatric cancer with features of skeletal muscle. Patel et al. show that intratumoral heterogeneity within rhabdomyosarcoma mimics the developmental states of embryonal myogenesis. Furthermore, treatment selects for cells in a mesoderm-like state that are vulnerable to EGFR inhibition. Combining EGFR inhibitors with chemotherapy therefore improves treatment outcomes.
  • Aspartate metabolism in endothelial cells activates the mTORC1 pathway to initiate translation during angiogenesis

    • Roxana E. Oberkersch,
    • Giovanna Pontarin,
    • Matteo Astone,
    • Marianna Spizzotin,
    • Liaisan Arslanbaeva,
    • Giovanni Tosi,
    • Emiliano Panieri,
    • Sara Ricciardi,
    • Maria Francesca Allega,
    • Alessia Brossa,
    • Paolo Grumati,
    • Benedetta Bussolati,
    • Stefano Biffo,
    • Saverio Tardito,
    • Massimo M. Santoro
    Oberkersch et al. demonstrate that endothelial cells (ECs) use glutamate and transaminase-mediated aspartate to drive angiogenesis in developmental and pathological angiogenesis. Mechanistically, aspartate drives mTORC1 activation that leads to the initiation of translation of endothelial growth factor receptors as well as pyrimidine synthesis in ECs.
  • Epithelial cells adapt to curvature induction via transient active osmotic swelling

    • Caterina Tomba,
    • Valeriy Luchnikov,
    • Luca Barberi,
    • Carles Blanch-Mercader,
    • Aurélien Roux
    Open Access
    Tomba et al. examine the response of epithelial cells to rapid changes in curvature. They show that upon curvature induction, cell volume transiently increases while membrane tension decreases and actin depolymerizes. Actin repolymerization and membrane tension recovery restore cell volume and forces in the new curved configuration.

Resources

  • High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae

    • Mingyue Wang,
    • Qinan Hu,
    • Tianhang Lv,
    • Yuhang Wang,
    • Qing Lan,
    • Rong Xiang,
    • Zhencheng Tu,
    • Yanrong Wei,
    • Kai Han,
    • Chang Shi,
    • Junfu Guo,
    • Chao Liu,
    • Tao Yang,
    • Wensi Du,
    • Yanru An,
    • Mengnan Cheng,
    • Jiangshan Xu,
    • Haorong Lu,
    • Wangsheng Li,
    • Shaofang Zhang,
    • Ao Chen,
    • Wei Chen,
    • Yuxiang Li,
    • Xiaoshan Wang,
    • Xun Xu,
    • Yuhui Hu,
    • Longqi Liu
    Open Access
    Using Stereo-seq, Wang et al. resolved the 3D spatial transcriptomes of Drosophila late-stage embryos and larvae with molecular and cellular dynamics patterns. This work opens the opportunity for the systematic study of gene regulatory networks during Drosophila development with organism-wide spatiotemporally resolved transcriptomic information.
  • Spatiotemporal mapping of gene expression landscapes and developmental trajectories during zebrafish embryogenesis

    • Chang Liu,
    • Rui Li,
    • Young Li,
    • Xiumei Lin,
    • Kaichen Zhao,
    • Qun Liu,
    • Shuowen Wang,
    • Xueqian Yang,
    • Xuyang Shi,
    • Yuting Ma,
    • Chenyu Pei,
    • Hui Wang,
    • Wendai Bao,
    • Junhou Hui,
    • Tao Yang,
    • Zhicheng Xu,
    • Tingting Lai,
    • Michael Arman Berberoglu,
    • Sunil Kumar Sahu,
    • Miguel A. Esteban,
    • Kailong Ma,
    • Guangyi Fan,
    • Yuxiang Li,
    • Shiping Liu,
    • Ao Chen,
    • Xun Xu,
    • Zhiqiang Dong,
    • Longqi Liu
    Open Access
    Liu et al. employ Stereo-seq to dissect the spatiotemporal transcriptomic dynamics in developing zebrafish embryos. This study provides a spatially resolved resource for the research of vertebrate embryogenesis and also helps to uncover how molecular and cellular interactions contribute to zebrafish embryogenesis.
  • The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves

    • Keke Xia,
    • Hai-Xi Sun,
    • Jie Li,
    • Jiming Li,
    • Yu Zhao,
    • Lichuan Chen,
    • Chao Qin,
    • Ruiying Chen,
    • Zhiyong Chen,
    • Guangyu Liu,
    • Ruilian Yin,
    • Bangbang Mu,
    • Xiaojuan Wang,
    • Mengyuan Xu,
    • Xinyue Li,
    • Peisi Yuan,
    • Yixin Qiao,
    • Shijie Hao,
    • Jing Wang,
    • Qing Xie,
    • Jiangshan Xu,
    • Shiping Liu,
    • Yuxiang Li,
    • Ao Chen,
    • Longqi Liu,
    • Ye Yin,
    • Huanming Yang,
    • Jian Wang,
    • Ying Gu,
    • Xun Xu
    Open Access
    Xia et al. establish the single-cell spatial transcriptome technique (scStereo-seq) in Arabidopsis leaves and discover subtle but significant transcriptional differences between cell subtypes, demonstrating that scStereo-seq is a powerful tool to integrate single-cell location and transcriptome information for plant biology study.
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